Column:

SRT-500, 5 µm, 500 Å , 7.8 x 300 mm

Mobile phase:

2 mM Potassium phosphate buffer + 10 mM NaCl ( pH 7.0 ) : ACN = 75 : 25 ( v/v )

Flow rate:

1.0 mL/min

Temperature:

25 ^oC

Detection:

UV 260 nm

Injection volume:

10 µL

Sample:

Humic acid ( HA ) test sample HA-A and Standard sample HA-Sigma ( 1 mg/mL )

1.HA-A

2.HA-sigma